Author: Jonathan Losos Page 2 of 128

I was recently reminded of the paper by Estrada and Silva Rodriguez on the ecological morphology of Cuban anoles. It was one of the first papers to quantitatively examine differences in morphology among a large and ecomorphologically diverse set of species. It was published in Ciencias Biológicas which can be hard to access, probably accounting for the fact that it hasn’t received the attention it deserves.

The picture and caption say it all. Read the paper by Felipe Espinoza De Janon and Mario Yánez-Muñoz.

So little is known about what anoles do high above the ground that information from tree climbing censuses is always welcome. Thomas et al. report on their observations of several anoles tens of meters off the ground in the Chocó region of northwestern Ecuador. 

We recently reported on the new U.S. Fish & Wildlife Service review of the status of A. roosevelti (spoiler alert: not good). And we mentioned that we’ve had a lot of posts on this species before, including a number of recountings of previous attempts to find the elusive beast. In turn, this post prompted Ray Huey to recount his own efforts. Here goes:

During my first summer at Harvard (1971), I joined Ernest Williams (my advisor) and two grad students (Preston Webster, Joan Roughgarden) on a trip to the Caribbean. This was my first introduction to the amazing Anolis lizards. We went to the Dominican Republic, Guadeloupe (magnificent), Puerto Rico, and then Culebra–discovered by Columbus–off the east coast of Puerto Rico.

In 1939, the U.S. Navy started using Culebra as a target for bombing practice. But is also the island where Anolis roosevelti, a “giant” anole”–was found, but last seen in 1931. Williams thought we should try to find the beast, even though it had probably been blown to extinction.

We arrived at the tiny town of Culebra Pueblo in late afternoon. The only hotel was full, but we found a small rental house run by Frank Slaughter. Frank was colorful.  He had been a boat captain and had ferried Richard Levins and Harold Heatwole around while they were doing biological surveys on small islands near Puerto Rico.

Slaughter had a way with words. He referred to Levins as a “strange duck”: “When I was in high school, people were counting the number of hairs on the asshole of fruit flies. And here was Richard Levins, a grown man, still counting the number of hairs on asshole of fruit flies.”

Given that Levins was one of the premier evolutionary theorists of his era, we all howled.

Back to the business of looking for Anolis roosevelti. Williams asked Slaughter whether he’d seen any big green lizards on Culebra. Slaughter replied, “How big is big? Do you have to reach up to milk it?” Williams (who was usually formal, especially with strangers) was speechless. The rest of us had to work to keep from laughing. All things are relative, but some are more relative than others.

You can access the report here.

Previous Anole Annals posts on A. roosevelti–some guaranteed interesting!–can be found here.

English translation now available!

 

Robert Hoogveld writes:

This year the Dutch society for Herpetology “Lacerta” celebrates its 80th anniversary. To celebrate, a lot of hard work has been done over the past year on a special edition of our journal, Lacerta, entirely aimed at anoles. We call it the Anolis special. It has become a 256-page book with contributions from Anolis enthusiasts and connoisseurs from the Netherlands, Belgium, Germany, Finland and Canada. It contains articles about, among other things, the study of anoles, behavior and welfare, travel reports with herpetological observations and species descriptions including the experiences of keeping in captivity.

At the moment the book can be ordered for anyone interested.  website: https://nvht-lacerta.nl/.On that website, everybody can order a copy for €20, including shipping world wide.

Dorothy Patent, an author who specializes in writing books for young readers about science and nature, has turned her attention to our favorite lizards. Lavishly illustrated with beautiful photos by the Day’s Edge team of Nate Dappen and Neil Losin, the book is a fabulous introduction to studying  evolution as it occurs.

Scientists featured will be familiar to AA readers: Shane Campbell-Staton, Jason Kolbe, Rob Pringle, James Stroud, Kristin Winchell and me.

A perfect stocking stuffer for kids of all ages (though 8-12 years old (3rd – 7th grade) is the target audience!

Here’s the description from Amazon:

In this groundbreaking, exceptionally researched installment of the award-winning Scientists in the Field series, discover how lizards rapidly adapt to life in the Caribbean islands, allowing scientists to study Charles Darwin’s theory of evolution by natural selection in real time. 

Award-winning author Dorothy Hinshaw Patent joins forces with scientists/filmmakers Neil Losin and Nate Dappen, whose work is detailed in the Smithsonian Channel documentary “Laws of the Lizard,” to explore how the small but mighty lizards we call “anoles” are used by scientists to study basic principles of evolution and ecology.

Travel with the team to Florida and the Caribbean as they research how anoles followed similar but independent evolutionary paths on the four major islands of the Greater Antilles (Puerto Rico, Hispaniola, Jamaica, and Cuba). So while anoles on different islands may look like close relatives, they often are not! This is Darwin’s principle of natural selection at work.

And it makes anoles the perfect subjects for experiments that study how animals adapt to new challenges—such as climate change—in this exciting and timely addition to a celebrated series.

 

Robert Hoogveld writes:

This year the Dutch society for Herpetology “Lacerta” celebrates its 80th anniversary. To celebrate, a lot of hard work has been done over the past year on a special edition of our journal, Lacerta, entirely aimed at anoles. We call it the Anolis special. It has become a 256-page book with contributions from Anolis enthusiasts and connoisseurs from the Netherlands, Belgium, Germany, Finland and Canada. It contains articles about, among other things, the study of anoles, behavior and welfare, travel reports with herpetological observations and species descriptions including the experiences of keeping in captivity. Although it is written in Dutch, I still think it is worth bringing this to your attention.

At the moment the book can be ordered for anyone interested. In 2023, every article will be translated into English and it will be made available on the Lacerta website: https://nvht-lacerta.nl/.On that website, everybody can order a copy for €20, including shipping world wide.

An Anole Annals reader writes:

I am in search of a green anole stuffed animal for my 6 year old, Luke. He is absolutely obsessed with lizards, and for Christmas this year, he wants a stuffed green anole.  He already has a stuffed Komodo dragon and stuffed chameleon which he loves.  We live in Texas, and he spends countless hours in our backyard, hunting for, catching, and playing with green anoles.  I never thought lizards could bring someone so much joy, but they certainly do for him!  He just turned 6 in November, and we spent the day feeding iguanas at the aquarium.  I have looked all over the internet, and it appears there used to be one made (pictured above; click link for Anole Annals story on it), but it was discontinued quite awhile ago and I’ve been unsuccessful in finding any used ones on ebay, etc.  If anyone has one they’d be willing to sell or part with, I would truly appreciate it, and it would make Luke so happy to open on Christmas morning. Thank you!

Mary can be reached at mary.feffer@gmail.com or 310-341-8592.

 

Postscript: Mary wrote a few days: “I just wanted to reach back out to you and let you know that thanks to your post, I was able to get the green anole stuffed animal which arrived in the mail today!  It is perfect, and I know Luke is going to love it on Christmas.  Thank you so much again for your help!”

 

From Science, September 22, 2022 

On a hot day in July, evolutionary biologist Martha Muñoz is leading four undergraduate students on a scouting expedition in the Smoky Mountains of North Carolina. As they hike up a steep trail, Muñoz turns over rocks and pokes leaf litter to assess where they might find salamanders when they return that night. She quizzes the students about how the weather might affect their chances, then demonstrates how the crunch of leaves underfoot is an easy way to assess an area’s dryness. Too much crunch means the salamanders won’t be out that night.

When one student falls behind, Muñoz hangs back to lend a hand if needed. Aha Anderson has a balance disorder and apologizes for their slowness. “No apologies needed,” Muñoz assures them. Later the crew will stop by Walmart to pick up a walking stick for Anderson. When another student, Jesús Buenrostro, proves squeamish about spiders, centipedes, and even grasshoppers, Muñoz reassures him with a few words in Spanish.

After dark, the group will return with headlamps, thermometers, and humidity sensors—and the goal of collecting 10 gray-cheeked salamanders to add to the growing salamander collection in Muñoz’s lab at Yale University. They’ll document the precise environment in which each one is found.

The southern Appalachians are a diversity hot spot for these creatures, but many of the roughly 30 species of lungless salamanders here look similar. Their environment also seems uniform, at least at first glance—creating a puzzle about how so many species could have evolved. Muñoz suspects subtle differences in behavior or habitat may have driven the salamanders to diversify, and she wants to figure out what they could be.

At 37, Muñoz has already won recognition for her discoveries about underappreciated influences on evolution, some of which buck classical thinking in the field. Her extensive studies with Caribbean lizards called anoles, for example, have provided some of the best empirical evidence that organisms can shape their evolutionary trajectory through their behavior, either speeding up or slowing down the evolution of physiological and morphological traits. She brings perspectives from multiple disciplines to evolutionary questions, says Robert Pringle, an evolutionary ecologist at Princeton University. “Her research is at the nexus of ecology, evolution, and physiology, and she has been in the vanguard of testing whether behavior acts as a drag on evolution or instead accelerates it,” Pringle says.

Muñoz sees a parallel in her own career path. The daughter of Cuban refugees, she knows firsthand the challenges people from underrepresented groups face as they try to get a toehold in academic science. “There is power in knowing that we can take control of our own circumstances, that we can guide our futures,” she says. “And there is even more power in knowing that this is a process that has unfolded for millions of years. It’s not the exception; it’s the norm.”

With that in mind, not only does Muñoz work hard to influence evolutionary thinking, she also strives to make sure others have a chance to make their own impact, no matter their background. “In my home you could often hear, ‘El éxito de uno de nosotros es el éxito de todos’—the success of any of us is a success for all of us,” she explains. “This is how I run my lab.”

MUÑOZ CREDITS her grandmothers and parents for her work ethic and success. After fleeing Cuba in the 1970s, her maternal grandmother scrubbed toilets to keep Muñoz’s mother and aunt housed and fed and later took care of Muñoz so her parents could work. The family eventually moved to a semidetached house in Queens near LaGuardia Airport, where despite regular insults from a racist neighbor, Muñoz found the diverse neighborhood exciting and inspiring. “We were all immigrants, all trying to get ahead,” Muñoz recalls. To help out, Muñoz took a job as a cashier at the local Rite Aid, where she endured threats from angry patients being refused expired prescriptions, met customers who had to choose between food and medicine, and put up with condescending doctors. “There isn’t anything about being a PI [principal investigator] that you can’t learn by being in retail,” she says.

Muñoz fell in love with nature at an early age. She and a friend scaled the chain link fence at a local park, pretending they were climbing trees in the wilderness. “I dragged every adult I could find” to the American Museum of Natural History, the Bronx Zoo, and the New York Botanical Garden, where she could connect to the natural world.

In freshman biology at Boston University, she learned about the rapid diversification of animal species during the Cambrian explosion more than 500 million years ago. It “moved me to tears,” she recalls, and inspired her to study evolutionary biology. She was accepted into a Ph.D. program at Harvard University, which had rejected her undergraduate and midcollege transfer applications. “I was so proud to be able to tell my parents I got into Harvard because then they relaxed—they knew they had done their part,” she says.

At Harvard, she worked with evolutionary biologist Jonathan Losos, whose research on Caribbean anoles has become a classic example of how evolution can follow a predictable path. For decades Losos and his students have studied lizards introduced to new islands, finding that when faced with similar challenges, these newcomers often adapt by evolving similar characteristics.

Muñoz added a twist to this story with field research on anoles in the Dominican Republic, which boasts some of the region’s highest peaks. Tropical lizards there can thrive at 3000 meters’ elevation, where it can be bitter cold. Most researchers had assumed that when a tropical lizard expands to the top of a mountain, its body would change over generations to tolerate the cold. But after comparing different species, Muñoz found little evidence of physiological differences that would confer cold tolerance. Instead, whereas sea-level anoles seek shelter from Sun in moist vegetation, the high-altitude lizards stayed warm by spending their days perched on boulders. They were “behaviorally nimble, exploiting Sun and shade to their advantage to stay optimally warm,” Muñoz explains.

The mountain lizards’ shift in behavior sped up morphological change, Muñoz found. Compared with their peers at low elevations, they had quickly evolved shorter hindlegs and flatter skulls that enabled them to hide from predators in narrow crevices in the rocks where they bask, she and her colleagues reported in 2017. The work showed a single behavior could slow one aspect of evolution, such as physiological changes in heat tolerance, and speed up another, such as the changes in anatomy she’d observed. “Far from being passive vessels at the mercy of their circumstances, organisms can influence evolution directly,” she says.

That idea wasn’t new, but prior to Muñoz few researchers had gone looking for empirical evidence. The influence of behavior on evolution “is an underemphasized problem that has not received nearly enough attention,” says Harry Greene, an emeritus evolutionary biologist at the University of Texas, Austin. With her data, “Muñoz is causing us older folks to think harder.”

After finishing her Ph.D., Muñoz did a postdoc at Duke University, where she explored another underappreciated influence on evolution: biomechanics. Duke integrative biologist Sheila Patek had been figuring out how predatory mantis shrimp evolved such fast, powerful forelimbs for crushing the shells of the snails they eat and snagging prey swimming by, and what influenced their evolutionary trajectories. These invertebrates use what’s called a four-bar linkage, in which components of the forelimb act (mechanically speaking) as four “bars” connected end to end via movable joints to form a closed loop that can resemble a parallelogram. This arrangement abounds in nature and in human-engineered devices, such as locking pliers. Many researchers had assumed each bar had a similar influence on the forces produced and would be equally likely to evolve.

But that’s not what Patek and Muñoz found. By comparing bar lengths in 36 species with known relationships on the mantis shrimp family tree, they showed the shortest bar often changed as a new species evolved. That bias most likely arose because the shortest bar has the most dramatic effect on mechanical output, amplifying force more than any of the other three when it got shorter.

Patek and Muñoz made a similar discovery in certain fish with four-bar linkages in their jaws. This arrangement enables wrasses, cichlids, and sunfish to snap open their mouths extra wide and suck in prey, and the proportions of the bars in these fish vary depending on whether their prey is fast moving or stationary. Fish that chase faster prey have shorter short bars that generate more force and enable them to snap prey faster, the researchers reported in 2018. Much like behavior, biomechanical principles can sculpt the rate, pattern, and direction of evolution, Muñoz says.

In 2020, Muñoz won the Society of Integrative and Comparative Biology’s award for achievements in biomechanics. The following year she won the society’s comparative physiology award, becoming the first researcher to win both. “She is able to integrate diverse concepts in novel and interesting ways, says Raymond Huey, an emeritus ecologist at the University of Washington, Seattle. “Most people focus on ‘A’ or ‘B,’ a few can add A plus B, but Martha can multiply them.”

IN 2019, Muñoz landed her current job at Yale, where ecology and evolutionary biology department chair Thomas Near has been working to recruit faculty from underrepresented groups and provide a welcoming environment. In his interview with Muñoz, Near acknowledged the challenges she’d face if she took the job. “He understood that I would have to battle the diversity dimension as well as the academic dimension,” she says.

These were challenges she knew well, having previously experienced the “imposter syndrome” common among scientists from underrepresented groups, who feel (however unjustly) that they don’t deserve to be where they are. She’d endured slights and insults as well, such as being told she’d have to work hard even though she was a diversity hire. The reality, Muñoz says, is that scientists from underrepresented groups feel tremendous pressure to work even harder than their peers. “We know that we have undue visibility due to our sparse numbers and correspondingly, we have a responsibility to be the best role models possible.”

At Yale, Muñoz signed up to be a resident fellow in one of the colleges, where undergraduates are housed, so she and Vigo, her German shepherd, would be embedded in the community. Seven months after arriving in New Haven, Connecticut, COVID-19 grounded her—and gave her time to write a proposal for the grant that now supports the salamander work.

The dozens of woodland-dwelling Plethodon species in the southern Appalachians posed irresistible evolutionary questions. These salamanders look so much alike, and the environment they live in seems so uniform, that researchers have considered them an example of “nonadaptive” radiation, in which organisms split into multiple species through the accumulation of random mutations and the slow march of geographic isolation, not because they have evolved different traits. Based on her work on lizards, Muñoz suspected there might be more to that story. Perhaps these salamanders have evolved behavioral or physiological differences that make each species distinctive, or perhaps their environment isn’t as uniform as it appears, creating subtle selective pressure to diversify.

Like about two-thirds of the 700 or so species of salamanders, Plethodon species lack lungs, breathing instead through their skin. Lungless salamanders have limited oxygen to fuel their activities and must make sure their skin stays moist enough to absorb as much oxygen from the air as possible. They’ve adapted by hiding and resting during the day, and by having a simplified nervous system to reduce their energy needs. As the evening cools down, they emerge from burrows, leaf litter, or rock crevices to sit, wait, and nab any insects or other prey that wander by. Most salamanders spend their lives within just a few square meters.

In the past few years Muñoz and her colleagues have collected thousands of observations of these animals, carefully recording the temperature and humidity at the exact spot where each salamander was spotted and at many other spots nearby. Already, they have documented diverse “microhabitats” in their study area—at the base of trees, under rhododendron leaves, on rocky ledges, and elsewhere—each with a specific range of temperature and humidity.

In a 2020 study of 26 species led by her postdoc Vincent Farallo, now at the University of Scranton, Muñoz and colleagues found that each prefers a slightly different combination of temperature and humidity. By choosing certain spots, each species is hydro- and thermoregulating, Muñoz says. Overall, the species mostly fall into two groups. One chooses warm, wet surroundings, where the moisture helps their skin absorb oxygen. “If their environment is wet, then they can capitalize on warmer temperatures,” which allows them to be more active, Muñoz explains. A second group can tolerate drier environments—but must opt for shade or other cooler places to keep from dying out.

Muñoz hosts hundreds of salamanders from dozens of species in her lab, where she and colleagues are measuring metabolic rates, water loss rates, preferred temperatures, heat tolerance, cold tolerance, and other traits. They hope to learn whether the animals’ preferences for specific spots, combined with physiological adaptations, may be contributing to the formation of new species.

So far they’ve found that resistance to water loss varies considerably among species, suggesting this physiological trait is evolving rapidly. Species that are less tolerant of water loss prefer wetter environments in the wild, whereas species that are more resistant to desiccation can use drier environments. If salamanders have chosen different microhabitats to suit their different moisture requirements, some populations could be becoming isolated from others, potentially setting the stage for them to become a new species.

AFTER WORKING IN THE LAB all summer, Muñoz’s students are eager to see the salamanders in their native habitat. The first night out is challenging, as the species they’re seeking proves elusive. But by the second night the students know the routine better, and they’ve set their sights on a different species that proves to be more plentiful. Anderson, with the aid of the new walking stick, catches a few to help the group meet its goal. And Buenrostro, who as a youth worked alongside his mom packing fruit, shows no fear as he digs into the dirt. They finish up before midnight, far earlier than expected. “You guys are awesome,” Muñoz says. “In one day, you figured it all out.”

Such encouragement is quintessential Muñoz, says Jessica Coutee, one of the students on the trip. Coutee, an Army veteran, admits she wasn’t sure what to make of Muñoz when they first met. Muñoz was wearing an elegant red dress as she led a group of veterans on a tour of Yale’s natural history museum. But she didn’t hesitate to don a pair of long yellow gloves and plunge her hands into a tub of chemicals to pull out a preserved giant iguana to show the group. “When you look at her, you might think she’s a girly girl, but she’s not,” Coutee says. Coutee, who calls herself Louisiana Creole as she’s a mix of Black, French, and Native American, is part of the first generation in her family to go to college. She, too, has wrestled with imposter syndrome, but not in Muñoz’s lab. “I feel I belong,” she says. “It’s an unbelievable feeling that I just don’t want to let go of.”

Providing a nurturing community for students of all backgrounds is Muñoz’s goal. “The first step into science is the hardest, so I try to make it as easy as possible,” she explains. Meanwhile, she’s still trying to figure some things out for herself. She is thinking about starting a family, but she has yet to receive tenure and still feels pressure to be perfect. “It feels as if I’m barely above water.”

Those closest to Muñoz say she works too hard, and she doesn’t deny it. But she says her work keeps her optimistic. “What nature is teaching us is that—like the lizards and salamanders I study—we are not passive vessels at the whim and mercy of our environments,” she says. “While we cannot extract ourselves from existing in a certain environmental context, I see hope and possibility in our future.”